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8 year oldWilliam and Catherine made the 30-minute journey from Victoria to Coal Harbour in British Columbia.
Thousands of people gathered on the waterfront to watch their arrival and cheered when the couple emerged from the Twin Otter aircraft.
It is first time the Cambridges have toured as a family of four, with Prince George and Princess Charlotte.
The royal couple were greeted by Canadian Prime Minister Justin Trudeau who was on the harbour side, before meeting well-wishers on a walkabout.
The duke and duchess were given earplugs for the flight because, although it is the easiest way to travel from Victoria to Vancouver, it is also the noisiest.
The tour began on Saturday when the Cambridges received a warm welcome from Canadians.
A royal aide said: "It was very smooth flying. The duke was very interested in the landing and the different conditions they work in. They spent a lot of time looking at the incredible views from both sides of the plane."
24 Sept Victoria, British Columbia: The duke and duchess, accompanied by their children, arrived in Victoria, the capital of British Columbia.
25 Sept Vancouver, British Columbia: The duke and duchess will visit Sheway, the Immigration Services Society of British Columbia - an event celebrating young leaders in Canadian arts, music, sport, charity, business, and film, and finally visit the Kitsilano coastguard station.
26 Sept Bella Bella and the Great Bear Rainforest, British Columbia: The duke and duchess will travel to the Great Bear Rainforest, visit the Heiltsuk First Nations community and attend a reception hosted by the province of British Columbia at Government House.
27 Sept Kelowna, British Columbia and Whitehorse, Yukon: The royals will tour the Okanagan campus of the University of British Columbia and take part in the BC government's "Taste of British Columbia" festival at Mission Hill Winery before flying to Whitehorse, where they will be greeted by members of the Canadian Rangers.
28 Sept Whitehorse and Carcross, Yukon: William and Catherine will visit the MacBride Museum and meet members of Whitehorse's cultural community before travelling to Carcross, where they will be welcomed by the Carcross/Tagish First Nation.
29 Sept Victoria, British Columbia: The royal couple and their children attend a children's party in the grounds of Government House, which will be attended by military families.
30 Sept Haida Gwaii, British Columbia: The duke and duchess visit Haida Gwaii, the archipelago on the northern coast of British Columbia, home to the Haida Nation. They will attend the opening of the new Haida Gwaii hospital and care centre. They will join local youths for a fishing expedition on the waters of Hecate Strait.
30 Sept Victoria, British Columbia: The royal couple will visit the Cridge Centre for the Family, which provides services and support for women who have experienced domestic violence. They will then meet families who have received support from the Kelty Mental Health Resource Centre and later youth working with the Sail and Life Training Society. They end their tour with a public official departure ceremony at Victoria Harbour Airport.