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7 year oldChief federal investigator Alexander Bastrykin and two men wanted in the UK for the murder of Russian dissident Alexander Litvinenko are among them.
The sanctions come amid worsening ties, including claims Russia ran a cyber campaign to influence the US election.
President-elect Donald Trump is seeking to restore closer relations with Russia.
US officials say the sanctions are not related to the hacking but come under a 2012 law designed to punish human rights violators.
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The move could be the last visible act against Russia by the outgoing administration, correspondents say.
Under the act named after Russian tax fraud whistleblower Sergey Magnitsky, who died in a Moscow prison in 2009, people on the list have their US assets blocked and are banned from travelling to the US.
It originally targeted officials implicated in Magnitsky's death but has since been broadened to cover other human rights cases.
The five men to be blacklisted are:
Litvinenko died after drinking tea laced with a rare radioactive substance at a hotel in London.
Both Mr Lugovoi and Mr Kovtun deny any involvement in the killing, and efforts to extradite the men to the UK have failed.
Last month, Washington expelled 35 Russian diplomats following allegations by US intelligence services that Russia had ordered the hacking of Democratic Party emails to damage Mr Trump's Democrat rival for the presidency, Hillary Clinton.
Russia denies the allegations and on Monday described them as a witch-hunt, but has so far not responded to the expulsions.
But after the 35 were thrown out Russia declined to respond in kind, with Russian President Vladimir Putin saying his country would not stoop to "irresponsible diplomacy".
Mr Trump, meanwhile, is said to have accepted the findings of the report but has declined to single out Russia as the source of the hacking.
<p>The two leaders have discussed the Ukraine conflict, with the German chancellor calling on Moscow to hold peace talks with Kiev</p>